thermo二抗A21207|Invitrogen抗體*

我們提供廣泛的Invitrogen™二抗結合物，具有良好的特異性特征，并使用多種優(yōu)質(zhì)熒光染料進行標記，包括Invitrogen™Alexa Fluor™熒光染料。 熒光二抗綴合物可用于其特定靶標的檢測，分選或純化，是熒光顯微鏡和共聚焦激光掃描顯微鏡，流式細胞儀和熒光西方檢測的理想選擇。 我們提供的熒光標記的廣泛性使我們的試劑可以適應幾乎任何熒光檢測系統(tǒng)。

可以以三種形式提供二抗：完整IgG，二價F（ab'）2片段和單價Fab片段。 由于許多免疫球蛋白結構域的結構具有高度保守性，因此大多數(shù)類特異性第二抗體必須經(jīng)親和純化和交叉吸附以實現(xiàn)與其他免疫球蛋白的小交叉反應。

我們的二抗結合物較常通過用來自目標物種的匯集的免疫球蛋白群免疫宿主動物來制備，并且可以進一步純化和修飾（例如，免疫親和層析，抗體片段化，標記綴合等）以產(chǎn)生高度特異性的試劑。。 在*輪純化中，回收與免疫抗體結合的完整免疫球蛋白，并且主要由~150-kDa IgG類組成。 進一步純化，例如，用蛋白A或G，除去與靶特異性免疫球蛋白重鏈和/或輕鏈反應的親和純化的抗體，除去所有不需要的免疫球蛋白類別。

thermo二抗A21207|Invitrogen抗體*

Product Specific Information

To minimize cross-reactivity, these donkey anti-rabbit IgG whole antibodies have been affinity-purified and show minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

Alexa Fluor染料是目前較值得信賴的熒光染料之一。 Invitrogen™Alexa Fluor 594染料是一種明亮的紅色熒光染料，具有理想的激發(fā)，適用于594 nm激光線。為了在成像和流式細胞儀中產(chǎn)生穩(wěn)定的信號，Alexa Fluor 594染料在很寬的摩爾范圍內(nèi)對pH不敏感。具有高熒光量子產(chǎn)率和高光穩(wěn)定性的探針允許以高靈敏度檢測低豐度生物結構。 Alexa Fluor 594染料分子可以高摩爾比附著在蛋白質(zhì)上而不會發(fā)生明顯的自猝滅，從而實現(xiàn)更明亮的結合物和更靈敏的檢測。每種綴合物的標記程度通常為每個IgG分子2-8個熒光團分子;每個產(chǎn)品批次的分析證書上都標明了確切的標簽程度。

使用結合物溶液：使用前在微量離心機中短暫離心蛋白質(zhì)結合物溶液;僅將上清液添加到實驗中。該步驟將有助于消除在儲存期間可能形成的任何蛋白質(zhì)聚集體，從而減少非特異性背景染色。由于染色方案隨應用而變化，因此應根據(jù)經(jīng)驗確定抗體的適當稀釋度。對于熒光團標記的抗體，對于大多數(shù)免疫組織化學和流式細胞術應用，終濃度1-10μg/ mL應該是令人滿意的。

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1-10 µg/mL

Immunocytochemistry (ICC)

2 µg/mL

Immunofluorescence (IF)

2 µg/mL

Immunohistochemistry (IHC)

1-10 µg/mL

Published Applications

Immunohistochemistry (IHC)

See 6 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 6 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 5 publications below

Immunocytochemistry (ICC)

See 2 publications below

Immunohistochemistry - Free Floating (IHC (Free))

See 1 publication below

Miscellaneous PubMed (MISC)

See 13 publications below

Product Specifications

Species Reactivity

Rabbit

Host / Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor® 594

Excitation/Emission Profile

View spectra 

Form

liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C, store in dark

RRID

AB_141637

Target

IgG

Antibody Form

Whole Antibody