Aurion BSA-Fragment Stabilized*

Aurion BSA-Fragment Stabilized

Aurion BSA-Fragment Stabilized*

Secondary antibodies have been thoroughly purified using solid phase technique. These purified antibodies allow detection of antigens with mouse monoclonal antibodies in rat tissue or rat monoclonal antibodies in mouse tissue, without causing interfering binding to either endogenous mouse or rat immunoglobulins.

The antibodies can be used for double labeling as well when both mouse and rat primary antibodies are used with other than mouse or rat tissue.

Please note:

Cross adsorption against closely related species may result in epitope recognition with reduced binding force and lower labeling density.

EMS and Aurion would like to inform you that the strongest labeling intensity is usually achieved with the least refined antibodies. Users are advised to take this into account when choosing a product from the Aurion range of anti-mouse or anti-rat conjugates. In case of doubt, our technical staff will gladly assist you in making the best choice.

Donkey Conjugate Line

In many cases double labeling experiments are based on using two primary antibodies from different animal species, for instance mouse and rabbit. Of late, we have noticed an increasing number of users that have primary antibodies developed in goat. It is impossible to perform a double labeling experiment in combination with, for instance a mouse primary antibody, since applicable secondary antibodies are either raised in rabbit or goat. Hence the Goat-anti-Mouse would be recognized by the Rabbit-anti-Goat, and it would be impossible to get reliable results. In the past, only using directly labeled primary antibodies would solve this. This would require researchers to go through the lengthy procedure of preparing such conjugates and even then with limited success.

To overcome this difficulty we are proud to release the Aurion Series of Secondary ImmunoGold conjugates based on antibodies raised in Donkey, including Donkey-anti-Goat. It is then known that mixing Donkey-anti-Goat and Donkey-anti-Mouse provides a successful solution to the above mentioned case.